In-vitro transcript MRNA and pharmaceutical composition comprising same
OwnerYoung Key Shin, Jun Young Choi +3inventors
InventorsYoung Key Shin, Jun Young Choi, Na Young Kim, Wonrak Son, Yong Jin Lee
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Abstract
The present invention relates to an RNA in-vitro transcript mRNA for intracellular expression of a gene of interest and a pharmaceutical composition comprising same for vaccines. When injected into animal cells, the in-vitro transcript mRNA including the gene of interest according to the present invention allows the protein of interest to be expressed in the animal cell in large quantities, and, as such, can be used as a gene vaccine against autoimmune diseases, infectious diseases, cancer- or tumor-related diseases, inflammatory diseases, and so on.
Claims10
An in-vitro transcript mRNA, comprising;
- (a) an RNA sequence insertion portion encoding a peptide of interest or a protein of interest;
- (b) 5′-UTR and 3′-UTR linked to both ends of an RNA sequence encoding the peptide of interest or the protein of interest;
- (c) a 5′ cap linked to 5′-UTR;
- (d) a poly (A) tail containing 20 to 400 adenines linked to 3′-UTR, and
- wherein the poly (A) tail contains uracil, in which the uracil is inserted between 2 to 20 adenines of the poly (A) tail, and an end of the poly (A) tail is adenine.
Dependencies are inferred from the claim text.