Methods for producing retinal tissue and retina-related cell
AssigneesSumitomo Chemical Company, Limited, Riken
InventorsTokushige Nakano, Satoshi Ando, Yoshiki Sasai, Mototsugu Eiraku
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Abstract
The invention provides a method for producing a retinal tissue by (1) subjecting pluripotent stem cells to floating culture in a serum-free medium containing a substance inhibiting the Wnt signal pathway to form an aggregate of pluripotent stem cells, (2) subjecting the aggregate to floating culture in a serum-free medium containing a basement membrane preparation, and then (3) subjecting the aggregate to floating culture in a serumcontaining medium. The invention also provides a method for producing an optic-cup-like structure, a method for producing a retinal pigment epithelium, and a method for producing a retinal layer-specific neural cell.
Claims12
An artificial cell aggregate consisting of a human retinal tissue, with Brn3 and TuJ1-positive ganglion cells in the lowermost layer of the retinal tissue, Crx and Recoverin-positive photoreceptor-precursor cells in the outermost layer and intermediate layer of the retinal tissue, and interneuron progenitor cells between the outermost layer and the lowermost layer of the retinal tissue, wherein the lowermost layer, intermediate layer, and outermost layer form a layered structure, and Crx and Recoverin-positive photoreceptor-precursor cells are accumulated in the outermost layer.
Dependencies are inferred from the claim text.