Hydrogel particle encapsulated viable cells for in vivo regenerative treatment
AssigneeCellDrop Biosciences, Inc.
InventorsWallace Chick, Benjamin Noren
Life of this patent
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Abstract
A composition for tendon and ligament regeneration includes a biologically compatible hydrogel suspension having spherical droplets. The spherical droplets have a diameter of about 100 μm to about 300 μm and a plurality of cells. The cells include marrow-derived mesenchymal stem cells (MSCs) with an average viability of about 90% or greater and cells that retain their stemness. A method for tendon and ligament regeneration includes forming the composition, purifying the composition, retrieving the composition, and administering the composition via injection into an injury site of injured tissue.
Claims6
A hydrogel composition for orthopedic tissue rejuvenation, the hydrogel composition comprising:
- a biologically compatible hydrogel suspension having hydrogel particles containing viable cells, wherein the hydrogel particles comprise:
- a PEG norbornene-based hydrogel polymer;
- the hydrogel polymer lacks cell-adhesive peptide groups;
- and
- wherein the hydrogel particles comprise spherical droplets having a diameter of about 100 μm to about 300 μm;
- wherein the hydrogel particles contain a total number of cells between about 15 cells to about 50 cells per hydrogel particle;
- wherein the viable cells within the hydrogel particles comprise an average viability of about 90% or greater;
- and
- wherein the biologically compatible suspension of hydrogel particles is devoid of suspension medium, such that the viable cells maintain viability of at least about 80% after 10 days and at least about 75% after two weeks.
Dependencies are inferred from the claim text.