IL-15 humanized mouse model and use thereof
AssigneeGempharmatech Co., Ltd.
InventorsCunxiang Ju, Weiwei Yu, Mingkun Zhang, Hongyan Sun, Dingyu Wang
Life of this patent
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Abstract
Provided are a mouse and a functional activity part thereof, comprising a humanized IL-15 gene; the humanized IL-15 gene comprises a human IL-15 gene segment and a mouse IL-15 gene segment, the human IL-15 gene segment comprises at least a part of exon 4, exon 5, exon 6, exon 7 and exon 8 of the human IL-15 gene, and the mouse IL-15 gene segment comprises exon 1, exon 2 and exon 3 of the mouse IL-15 gene. Also provided are a preparation method and use of the mouse.
Claims16
A mouse, comprising a humanized Interleukin-15 (IL-15) gene, wherein the humanized IL-15 gene comprises a human IL-15 gene segment and a mouse IL-15 gene segment,
- wherein the human IL-15 gene segment comprises a portion of exon 4, exon 5, exon 6, exon 7, and a portion of exon 8 of the human IL-15 gene,
- wherein the human IL-15 gene segment comprises the nucleic acid sequence of SEQ ID NO:
- 1, and
- wherein the mouse IL-15 gene segment comprises exon 1, exon 2 and exon 3 of the mouse IL-15 gene.
A method for preparing a genetically modified mouse, comprising the steps of:
- (a) modifying a fertilized mouse egg or a mouse embryonic stem (ES) cell by replacing a mouse interleukin-15 (IL-15) gene segment encoding a mature mouse IL-15 polypeptide on an endogenous mouse IL-15 locus with a human IL-15 gene segment encoding a mature human IL-15 polypeptide so as to form a humanized IL-15 gene, wherein the human IL-15 gene segment comprises a portion of exon 4, exon 5, exon 6, exon 7, and a portion of exon 8 of the human IL-15 gene, and wherein the human IL-15 gene segment comprises the nucleic acid sequence of SEQ ID NO:
- 1;
- (b) obtaining a modified fertilized mouse egg or ES cell from step (a);
- and (c) generating a mouse from the modified fertilized egg or ES cell obtained in step (b).
Dependencies are inferred from the claim text.