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US 12,580,106 B2Granted
Process for preparation of silica coated magnetic particles
AssigneeNorweigan University of Science and Technology
InventorsPer Arne Aas, Lars Hagen, Sten Even Erlandsen, Magnar Bjørås, Vegar Ottesen, Anuvansh Sharma, Sulalit Bandyopadhyay
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Abstract
The present invention relates to methods and products for isolating nucleic acids from samples containing biological material. In particular, the present invention relates to silica-coated magnetic particles, processes for their preparation and their use in methods of isolating nucleic acids samples containing biological material.
Claims12
A process for the preparation of a silica coated magnetic particles comprising
- (I) combining magnetic particles and an alkoxysilane in a C1-4-alcohol in the absence of water to form a mixture;
- (II) adding water and a hydroxide to the mixture of step (I) such that the weight ratio of C1-4-alcohol to water in the mixture is 1:1 to 15:1 and heating the resulting mixture to a temperature in the range of 15 to 90° C. in order to form silica coated magnetic particles;
- (III) washing the silica coated magnetic particles of step (II) with water and/or alcohol solvent, until the pH of the silica coated magnetic particles when suspended in water is between 8 and 11.
Dependencies are inferred from the claim text.