Spatially co-registered genomic and imaging (SCORGI) data elements for fingerprinting microdomains

A method of generating a plurality of spatially co-registered data elements from a tissue sample, the method comprising:

• obtaining a core from the tissue sample;
• obtaining from the core a plurality of imaging data sections and a plurality of genomic data sections in an alternating fashion along a length of the core such that each of the imaging data sections is associated with a respective one of the genomic data sections to form a plurality of serial pairs of adjacent, co-registered tissue sections from the core of the tissue sample, wherein for each serial pair of co-registered tissue sections from the core of the tissue sample, the imaging data section and the genomic data section are obtained from immediately adjacent portions of the core along the length of the core;
• for each serial pair of co-registered tissue sections from the core of the tissue sample:
• (i) obtaining and storing as part of a data element a plurality of biomarker labeled images obtained from the imaging data section of the serial pair of co-registered tissue sections from the core of the tissue sample by repeated labeling with a plurality of biomarker tags, (ii) generating and storing image data obtained from the plurality of biomarker labeled images as part of the data element, and (iii) generating and storing genomic data obtained from the genomic data section of the serial pair of co-registered tissue sections from the core of the tissue sample as part of the data element.

A system for generating and storing a plurality of spatially co-registered data elements from a plurality of imaging data sections and a plurality of genomic data sections obtained from a core of a tissue sample in an alternating fashion along a length of the core such that each of the imaging data sections is associated with a respective one of the genomic data sections to form a plurality of serial pairs of adjacent, co-registered tissue sections from the core of the tissue sample, wherein for each serial pair of co-registered tissue sections from the core of the tissue sample, the imaging data section and the genomic data section are obtained from immediately adjacent portions of the core along the length of the core, the system comprising a processing apparatus including:

• an image data generation component that is structured and configured to generate for each serial pair of co-registered tissue sections from the core of the tissue sample image data from a plurality of biomarker labeled images obtained from the imaging data section of the serial pair of co-registered tissue sections from the core of the tissue sample by repeated labeling with a plurality of biomarker tags;
• a region of interest identification component that is structured and configured to identify for each serial pair of co-registered tissue sections from the core of the tissue sample a number of regions of interest in the genomic data section of the serial pair of co-registered tissue sections from the core of the tissue sample based on the generated image data of the serial pair of co-registered tissue sections from the core of the tissue sample, wherein for each serial pair of co-registered tissue sections from the core of the tissue sample genomic data is generated from the genomic data section of the serial pair of co-registered tissue sections from the core of the tissue sample based on the identified number of regions of interest;
• and
• a data element generation component that is structured and configured to generate and store for each pair of co-registered tissue sections from the core of the tissue sample a spatially co-registered data element of the plurality of spatially co-registered data elements from the core of the tissue sample, the stored spatially co-registered data element including the plurality of biomarker labeled images, the generated image data, and the generated genomic data of the serial pair of co-registered tissue sections from the core of the tissue sample.