Systems and methods for glycopeptide concentration determination, normalized abundance determination, and LC/MS run sample preparation
AssigneeVenn Biosciences Corporation
InventorsDaniel Serie, Gege Xu
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Abstract
Embodiments described herein generally relate to systems and methods for processing mass spectrometry samples. Aspects of the disclosure include systems and methods for processing samples. Additionally, embodiments of the disclosure can also include systems and methods for sample analysis. Various embodiments include data analysis systems and methods for comparing data across samples and sample runs. Data analysis systems can run normalization methods for normalizing raw abundance mass spectrometry data. In some aspects, the normalized data can be used as input for predictive models.
Claims9
A method of determining a concentration of a target glycopeptide analyte in a sample, the method comprising:
- measuring an abundance for a non-glycosylated endogenous peptide (NGEP) of a sample;
- determining a corrected NGEP abundance for the NGEP using an external standard;
- measuring an internal standard abundance for an internal standard having a known internal standard concentration;
- determining an NGEP concentration for the NGEP as a function of the corrected NGEP abundance, the known internal standard concentration, and the internal standard abundance;
- determining a normalized abundance of a target glycopeptide analyte of the sample as a function of a measured abundance of the target glycopeptide analyte and the measured abundance of the NGEP on the same protein as the target glycoprotein analyte;
- and
- determining a target glycopeptide analyte concentration as a function of the NGEP concentration and the normalized abundance.
Dependencies are inferred from the claim text.