Determination of canine TK1 protein levels
AssigneeAlertix Veterinary Diagnostics AB
InventorsStaffan Eriksson, Henrik Rönnberg, Kiran Kumar Jagarlamudi
Life of this patent
Estimating the term…
Abstract
A kit useful for determination of canine TK1 in a sample is disclosed. The kit comprises a first monoclonal antibody, or a fragment thereof, immobilized to a support or intended to be immobilized to a support. The kit also comprises a second monoclonal antibody, or a fragment thereof. One of the monoclonal antibodies has specificity for a peptide consisting of an amino acid sequence from a C-terminal region of canine TK1 and the other monoclonal antibody has specificity for a peptide consisting of an amino acid sequence from an active site of TK1.
Claims17
A kit for determining a level of canine thymidine kinase 1 (TK1) protein in a sample, comprising:
- two monoclonal antibodies or fragments thereof, either one of which is immobilized to a support;
- wherein
- (a) one of the two monoclonal antibodies or the fragment thereof comprises:
- a variable heavy (VH) complementary determining region 1 (CDR1) having amino acid sequence SEQ ID NO:
- 19,
- a VH CDR2 having amino acid sequence SEQ ID NO:
- 20,
- a VH CDR3 having amino acid sequence SEQ ID NO:
- 21,
- a variable light (VL) CDR1 having amino acid sequence SEQ ID NO:
- 22,
- a VL CDR2 having amino acid sequence SEQ ID NO:
- 23, and
- a VL CDR3 having amino acid sequence SEQ ID NO:
- 24;
- and
- (b) the other of the two monoclonal antibodies or the fragment thereof comprises
- a VH CDR1 having amino acid sequence SEQ ID NO:
- 37,
- a VH CDR2 having amino acid sequence SEQ ID NO:
- 38,
- a VH CDR3 having amino acid sequence SEQ ID NO:
- 39,
- a VL CDR1 having amino acid sequence SEQ ID NO:
- 40,
- a VL CDR2 having amino acid sequence SEQ ID NO:
- 41, and
- a VL CDR3 having amino acid sequence SEQ ID NO:
- 42.
Dependencies are inferred from the claim text.