Sensor for detecting biomarkers in a fluid sample and methods of use

A diagnostic platform for detection of at least one biomarker in a fluid sample comprising:

• a quantitative sensing device configured to receive the fluid sample, wherein the fluid sample comprises blood, blood serum, or plasma;
• an electronic reading platform;
• and
• a computing device;
• wherein the sensing device comprises
• a layer of piezoelectric material comprising two faces;
• at least one electrode layer, wherein the at least one electrode layer is affixed to one face of the piezoelectric material, wherein the at least one electrode layer comprises
• a thickness of about 33 nm to about 300 nm, and
• a diameter of about 150 μm to about 350 μm;
• a second reference electrode layer that is affixed to a second face of the piezoelectric material;
• at least two disposable sensor cartridges, wherein one of the at least two sensor cartridges comprises a means for detecting IgG antibodies in the fluid sample and the other sensor cartridge comprises a means for detecting IgM antibodies in the fluid sample, wherein the IgG and IgM antibodies are quantitively detected;
• a Q-factor of at least 10,000;
• an operating frequency of about 10 MHz to about 90 MHz;
• and
• a sensing layer disposed upon the at least one electrode layer, wherein the sensing layer is configured to bind the at least one biomarker for a disease or condition, wherein the biomarker comprises an IgG antibody, an IgM antibody, or a combination thereof;
• wherein the quantitative sensing device is communicatively linked with the electronic reading platform,
• wherein the electronic reading platform is configured to receive sensor data from the sensing device and to communicate the data to the computing device,
• and wherein the computing device is configured to determine the presence, absence, or amount of the at least one biomarker;
• and
• wherein the diagnostic platform quantitatively detects the at least one biomarker within about 10 minutes.