Monolayer of PBMCs or bone-marrow cells and uses thereof

A method for determining whether a subject suffering from or predisposed to a disease will respond or is responsive to treatment with a therapeutic agent comprising:

• isolating bone-marrow cells from a bone-marrow sample obtained from the subject;
• generating, utilizing gravitational forces without centrifugation and on a culture device not treated or coated to promote cell adhesion, a monolayer of bone-marrow cells from the bone-marrow cells by incubating the bone-marrow cells in a solution exposed to the gravitational forces without centrifugation at a density of about 100 cells per mm2 to about 30000 cells per mm2 to maintain naturally occurring cell-cell interactions and membrane integrity;
• contacting the monolayer of bone-marrow cells that is not exposed to centrifugation with a therapeutic agent;
• and
• assessing a response of the monolayer of bone-marrow cells to the therapeutic agent by microscopic analysis of the monolayer of bone-marrow cells.