High throughput stochastic bio-molecular sensor
AssigneeMassachusetts Institute of Technology
InventorsSwathi Manda, Ashwin Gopinath
Life of this patent
Estimating the term…
Abstract
What is described herein is a device for sensing a target, comprising a planar array of unique stochastic sensors, wherein each sensor is weakly cross-reactive with a unique determinant on the target; a means for capturing electrical signals from each sensor and the temporal duration of each signal; and a means for analyzing the cumulative signals from the array of stochastic sensors, and optionally further comprising a computer system for processing an algorithm for identifying the target based on the electrical signals from the sensors of the device.
Claims13
A device for sensing one or more targets in a sample, comprising
- a bioprotonic conducting material forming a planar array of protodes on a non-conducting substrate, wherein each protode in the planar array of protodes is electrically isolated and individually addressable;
- a plurality of DNA origami tethered DNA nanopores immobilized to the planar array of protodes, wherein the outer surface of each of the plurality of DNA origami tethered DNA nanopores comprises one or more hydrophobic moieties;
- an insulating membrane, defining a space inferior to the membrane and a space superior to the membrane, wherein each of the DNA origami tethered DNA nanopores spans the membrane and provides an ionic pathway between the bioprotonic conducting material inferior to the membrane and bulk solution;
- a power supply in electrical contact with each protode to provide an electric potential difference across the membrane;
- and
- a detector, configured to independently acquire and resolve signals in real-time from each protode in the planar array of protodes, to detect changes in electrical signal through the DNA origami tethered DNA nanopore over time as each DNA origami tethered DNA nanopore interacts with the one or more targets, wherein the changes in electrical signal can comprise changes in signal magnitude, signal duration, or frequency of signal changes, and wherein signals from individual protodes are independently resolved to discriminate one or more targets in the sample.
Dependencies are inferred from the claim text.