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US 12,578,325 B2Granted
Staining method for live-cell imaging
Ownerhuimin Zhanginventor
Life of this patent
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Abstract
The present invention provides a fluorescent staining method for live-cell imaging. First, cells are double stained through the first and second fluorescent biomarker. Then, the clear fluorescent cell image is shown under a fluorescent microscope, and we can observe the nucleus form while observing the cell form through the obtained image.
Claims15
A staining method for live-cell imaging, wherein said method includes the following steps:
- simultaneously or separately (i) using a first fluorescent biomarker to stain target cells, which are live cells;
- (ii) using a second fluorescent biomarker, which is different from the first fluorescent biomarker, to stain the target cells;
- (iii) using a fluorescent microscope to obtain fluorescent images of the target cells, which can observe the nucleus form while observing the cell contour;
- wherein the first biomarker and the second biomarker are selected to ensure that, when using a fluorescent microscope to obtain the fluorescent images of the target cells, the first biomarker enters an exciting state after absorbing incident light energy and emits a first light to show the cell contour in the fluorescent images, and the second biomarker absorbs the first light to show the nucleus form in the fluorescent images.
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