Polypeptide nanopores synthetically functionalized with positively charged species, and methods of making and using the same
AssigneeIllumina, Inc.
InventorsLisa Savagian, Burton Simpson, Sang Park, Boyan Boyanov, Jeffrey G. Mandell, Seth M. McDonald
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Abstract
Polypeptide nanopores synthetically functionalized with positively charged species, and methods of making and using the same, are provided herein. In some examples, a polypeptide nanopore includes a first side, a second side, a channel extending through the first and second sides, and a mutated amino acid residue. The mutated amino acid residue may be synthetically functionalized with a positively charged species that inhibits translocation of cations through the channel.
Claims18
A polypeptide nanopore comprising a first side, a second side, a channel extending through the first and second sides, and a mutated amino acid residue which is synthetically functionalized with a positively charged species that inhibits translocation of cations through the channel, wherein the positively charged species has a net charge of +2, wherein the mutated amino acid residue comprises a natural amino acid residue or an unnatural amino acid residue, and wherein the unnatural amino acid residue comprises an alkyne, azide, or alkene.
A polypeptide nanopore comprising a first side, a second side, a channel extending through the first and second sides, and a mutated amino acid residue which is synthetically functionalized with a positively charged species that inhibits translocation of cations through the channel, wherein the polypeptide nanopore comprises MspA, and the mutated amino acid residue is located at residue 90, 91, or 93.
A polypeptide nanopore comprising a first side, a second side, a channel extending through the first and second sides, and a mutated amino acid residue which is synthetically functionalized with a positively charged species that inhibits translocation of cations through the channel, wherein the positively charged species comprises a cationic metal coordination complex.
A polypeptide nanopore comprising a first side, a second side, a channel extending through the first and second sides, and a mutated amino acid residue which is synthetically functionalized with a positively charged species that inhibits translocation of cations through the channel, wherein the positively charged species comprises an ionophore.
A polypeptide nanopore comprising a first side, a second side, a channel extending through the first and second sides, and a mutated amino acid residue which is synthetically functionalized with a positively charged species that inhibits translocation of cations through the channel, wherein the polypeptide nanopore comprises multiple polypeptide subunits, and wherein at least one of the multiple polypeptide subunits does not comprise the mutated amino acid residue.
A polypeptide nanopore comprising a first side, a second side, a channel extending through the first and second sides, and a mutated amino acid residue which is synthetically functionalized with a positively charged species that inhibits translocation of cations through the channel, wherein the polypeptide nanopore comprises multiple polypeptide subunits, and wherein at least two of the polypeptide subunits are cross-linked to one another.
A polypeptide nanopore comprising a first side, a second side, a channel extending through the first and second sides, and a mutated amino acid residue which is synthetically functionalized with a positively charged species that inhibits translocation of cations through the channel, wherein the positively charged species has a net charge of +2, wherein the polypeptide nanopore comprises multiple polypeptide subunits, and wherein each of the polypeptide subunits is coupled to a respective positively charged species.
Dependencies are inferred from the claim text.