Method for recovering biological substance and device for recovering biological substance

A method for recovering a biological substance using an electrophoresis device having a separation medium and a recovery hole and using an electric field, wherein, in the separation medium, a cross-sectional area of an outflow portion from which the biological substance flows out taken along a plane orthogonal to a direction of the electric field is smaller than a cross-sectional area of an inflow portion into which the biological substance flows taken along the plane orthogonal to the direction of the electric field,

• the method for recovering the biological substance comprising:
• injecting an injecting solution containing the target biological substance into an injection hole;
• a voltage application start step of starting application of a voltage for moving the biological substance toward the recovery hole;
• a buffer solution removing step of removing buffer solution in the recovery hole before the biological substance reaches the recovery hole after the voltage application start step;
• a solvent injection step of injecting a solvent into the recovery hole after the buffer solution removing step;
• a recovery step of recovering the biological substance that reached the recovery hole after the solvent injection step;
• and
• a cleaning step of cleaning the recovery hole using cleaning liquid between the buffer solution removing step and the solvent injection step;
• wherein in at least a part of the separation medium, a cross-sectional area having a height and width taken along the plane orthogonal to the direction of the electric field decreases in height and width two axes in a direction from the inflow portion toward the outflow portion.