Kits and methods for determining copy number of mouse TCR gene
AssigneeShanghai AbelZeta Ltd.
InventorsJiaqi Huang, Yihong Yao, Lin Guo, Jiaxu Yan, Xin Yao, Shigui Zhu, Fei Wang, Junfeng Wu, Jishun Lu, Jiaqiang Ren, Li Zhang
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Abstract
The present disclosure provides systems, kits and methods for determining the copy number of a mouse TCR transgene. The primers are specific to the conserved regions of the mouse TCR gene. The primers/probes provide good amplification efficiency and can quickly and accurately determine the copy number of a mouse TCR transgene.
Claims13
A system for determining a copy number of a mouse T cell receptor (TCR) gene, the system comprising
- (i) a forward primer comprising the nucleic acid sequence set forth in SEQ ID NO:1 and (ii) a reverse primer comprising the nucleic acid sequence set forth in SEQ ID NO:2, and (iii) a probe comprising a detectable label.
A kit for determining a copy number of a mouse T cell receptor (TCR) gene, the kit comprising:
- (i) a forward primer comprising a nucleic acid sequence set forth in SEQ ID NO:1 and (ii) a reverse primer comprising a nucleic acid sequence set forth in SEQ ID NO:2, and (iii) a probe comprising a detectable label.
The method of 9, wherein the nucleic acid amplification reaction is polymerase chain reaction (PCR).
Dependencies are inferred from the claim text.