Devices and method for detecting an amplification event

A method for detecting an amplification reaction in a solution containing a biological sample using an array of ion sensors, a processor operably coupled to the array of ion sensors, and a memory operably coupled to the processor, the amplification reaction being indicative of a presence of a nucleic acid, the method comprising:

• receiving, by the processor and throughout an amplification reaction, a signal from each respective sensor of the array of ion sensors;
• storing, by the processor, each signal received from each respective sensor of the array of ion sensors in the memory;
• detecting, by the processor and in real time, a change in the signal from a first sensor of the array of ion sensors by comparing a current signal value received from the first sensor to at least one previously obtained signal valve received from the first sensor and stored in the memory;
• comparing, by the processor and upon detecting a change in the signal from the first sensor, the signal from the first sensor with the signal of at least one neighboring sensor, the at least one neighboring sensor being proximate to the first sensor in the array wherein comparing the signal from the first sensor with the signal of the at least one neighboring sensor comprises calculation of a correlation parameter between the signal of the first sensor and the signal of the at least one neighboring sensor;
• and
• determining, by the processor and based on the correlation parameter, that an amplification event has occurred in the solution in the vicinity of the first sensor.