NAD(P)-dependent responsive enzymes, electrodes and sensors, and methods for making and using the same
AssigneeAbbott Diabetes Care Inc.
InventorsTianmei Ouyang, Benjamin J. Feldman
Life of this patent
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Abstract
NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.
Claims37
An electrochemical analyte sensor comprising a first portion configured to be positioned above a user's skin and a second portion configured to be transcutaneously positioned in the user's skin and in contact with the user's biological fluid to monitor the level of an analyte in vivo, the second portion comprising:
- i) an electrode;
- ii) an enzyme composition disposed upon the electrode, the enzyme composition comprising:
- a) nicotinamide adenine dinucleotide (phosphate) (NAD(P)+) or derivative thereof;
- b) D-3-hydroxybutyrate dehydrogenase;
- c) diaphorase;
- d) a polymeric redox mediator, and
- iii) a membrane disposed on the enzyme composition,
- wherein at least one of the D-3-hydroxybutyrate dehydrogenase and the diaphorase is covalently bonded to the polymeric redox mediator, and
- wherein the NAD(P)+ or derivative thereof is not covalently bonded to the polymeric redox mediator.
An electrochemical analyte sensor comprising a first portion configured to be positioned above a user's skin and a second portion configured to be transcutaneously positioned in the user's skin and in contact with the user's biological fluid to monitor the level of ketones in vivo, the second portion comprising:
- i) an electrode;
- ii) an enzyme composition disposed upon the electrode, the enzyme composition comprising:
- a) nicotinamide adenine dinucleotide (phosphate) (NAD(P)+) or derivative thereof,
- b) D-3-hydroxybutyrate dehydrogenase;
- c) diaphorase;
- d) an electron transfer agent;
- and
- e) a polymer;
- and
- iii) a membrane disposed on the enzyme composition,
- wherein at least one of the D-3-hydroxybutyrate dehydrogenase and the diaphorase is covalently bonded to the polymer, and
- wherein the NAD(P)+ or derivative thereof is not covalently bonded to the polymer.
Dependencies are inferred from the claim text.