Compositions and methods for peptide production
AssigneeBioCatalyst LLC
InventorsJamshid Davoodi, Mohammed M. Davoodi
Life of this patent
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Abstract
This disclosure concerns production of product peptides with a target amino acid sequence by proteolysis of a recombinant polypeptide comprising specific protease recognition sites or chemical cleavage sequences. In some embodiments, the product peptide is released from repeating peptide units in the recombinant polypeptide by removal of intervening amino acid sequences by proteolysis by proteases that recognize sites within the intervening amino acid sequences and a carboxypeptidase, aminopeptidase, and/or further protease.
Claims25
A nucleic acid sequence encoding the recombinant protein, the recombinant protein comprising a product peptide and one or more repeating units, each of the one or more repeating units comprising a chemical cleavage site, a protease cleavage site and a product peptide, wherein the chemical cleavage site is an amino acid sequence and is a site that leaves no amino acid at the P1 position upon cleavage;
- wherein the protease cleave site is an amino acid sequence and is a site that leaves no amino acid at the P1′ position upon cleavage, the recombinant protein arranged according to formula I
- PP1-[CCS-PCS-PP2]n (I)
- wherein
- brackets indicate a repeating unit of the one or more repeating units;
- PP1 is the first product peptide;
- PP2 is the second product peptide;
- CCS is the chemical cleavage site;
- PCS is the protease cleavage site;
- and
- n is an integer from 1 to 150.
A nucleic acid sequence encoding the recombinant protein, the recombinant protein comprising a first product peptide and one or more repeating units, each of the one or more repeating units comprising in linear order a chemical cleavage site, a protease cleavage site and a second product peptide, wherein the one or more repeating units, wherein the chemical cleavage site is an amino acid sequence and is a site that leaves no amino acid at the P1 position upon cleavage;
- wherein the protease cleave site is an amino acid sequence and is a site that leaves no amino acid at the P1 position upon cleavage.
Dependencies are inferred from the claim text.