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US 12,577,600 B2Granted
Modification of RNA-related enzymes for enhanced production
AssigneeTranslate Bio, Inc.
InventorsBettina Strack-Logue, Frank DeRosa, Michael Heartlein
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Abstract
The present invention provides, among other things, methods and compositions for large-scale production of capped mRNA using SUMO-Guanylyl Transferase fusion protein.
Claims4
A method of producing a capped RNA or RNA analog oligonucleotide, comprising transferring and methylating a guanylyl molecule to the 5′ end of the RNA or RNA analog oligonucleotide using a guanylyl transferase, wherein the guanylyl transferase comprises
- i) a fusion protein comprising a small ubiquitin-like molecule (SUMO) protein covalently linked to guanylyl transferase large subunit, wherein the SUMO protein comprises SEQ ID NO:
- 1 and the guanylyl transferase large subunit comprises SEQ ID NO:
- 6, and
- ii) a guanylyl transferase small subunit comprising SEQ ID NO:
- 7, thereby producing a capped RNA or RNA analog oligonucleotide.
2 more elements
Dependencies are inferred from the claim text.