Compositions and methods for mammalian genetics and uses thereof
AssigneeWhitehead Institute for Biomedical Research
InventorsThijn R. Brummelkamp, Jan E. Carette
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Abstract
The invention provides compositions and methods for performing mammalian cell genetics, e.g., genetic screens, using near-haploid cells. The invention further provides genes and gene products isolated using the inventive methods and methods of use thereof.
Claims6
An adherent, near-haploid mammalian cell derived from a non-adherent near-haploid KBM7 cell, wherein the non-adherent near-haploid KBM7 cell comprises viral vectors encoding Oct4, Sox2, Klf4 and c-Myc.
A method of deriving an adherent, near-haploid KBM7 cell comprising (a) introducing viral vectors encoding Oct4, Sox2, Klf4, and c-Myc into a non-adherent, near-haploid KBM7 cell in culture;
- and (b) maintaining said culture for a time sufficient for formation of an adherent near-haploid KBM7 derived cell , wherein the adherent, near-haploid KBM7 cell comprises exogenous DNA encoding Oct4, Sox2, Klf4 and c-Myc.
Dependencies are inferred from the claim text.