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US 12,577,584 B2Granted
Non-HLA matched humanized NSG mouse model with patient-derived xenograft
AssigneeThe Jackson Laboratory
InventorJames Keck
Life of this patent
Estimating the term…
Abstract
The invention described herein provides non-HLA matched humanized mouse model (e.g., NSG mouse model) with patient-derived xenograft (PDX), as well as methods of making and using the same.
Claims12
A method of producing a mouse model for in vivo preclinical testing, the method comprising:
- (a) introducing CD34+ human hematopoietic stem cells (HSCs) into an immunodeficient non-obese diabetic mouse comprising a homozygous scid mutation and an IL-2 receptor gamma chain deficiency to produce a humanized mouse in which the percentage of human CD45+ cells in peripheral blood is about 20-30%;
- and
- (b) introducing into the humanized mouse a human PDX about 9, 10, 11, 12, 13, 14, or 15 weeks after introducing the CD34+ human HSCs, wherein the human PDX and the CD34+ human HSCs are non-HLA matched, the human PDX develops into a tumor, and the CD34+ human HSCs mature into human CD19+ B cells and human CD8+ T cells to produce a humanized mouse engrafted with the human tumor.
Dependencies are inferred from the claim text.