Methods and compositions for promoting non-natural amino acid-containing protein production
AssigneeAmbrx, Inc.
InventorsSigeng Chen, Yingchun Lu, Feng Tian
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Abstract
Disclosed herein are methods and compositions for generation of cell lines to promote unnatural amino acid-containing protein production using genome engineering technology.
Claims27
A method of generating a cell line for incorporating a non-natural amino acid into a protein, the method comprising inactivating one or more target site or region in a cell, wherein the one or more target site or region is Bak, Bax, or both;
- wherein the cell expresses a selector codon containing gene of interest, and wherein the cell comprises an orthogonal aminoacyl tRNA synthetase and an orthogonal suppressor transfer RNA (tRNA).
A cell or cell line for incorporating a non-natural amino acid into a protein, wherein the cell or cell line expresses a selector codon-containing gene of interest, wherein the cell or cell line comprises an orthogonal aminoacyl tRNA synthetase, and an orthogonal suppressor tRNA;
- and wherein the cell or cell line comprises one or more inactivated target site or region, wherein the one or more inactivated target site or region is Bak, Bax, or both.
A method comprising:
- (i) generating a cell or cell line for incorporating a non-natural amino acid into a protein, comprising:
- providing a cell or cell line expressing a selector codon-containing gene of interest, and comprising an orthogonal aminoacyl tRNA synthetase and an orthogonal suppressor tRNA;
- and
- inactivating one or more target site or region in the cell or cell line, wherein the one or more target site or region is Bak, Bax, or both;
- and
- (ii) producing the protein comprising the non-natural amino acid.
Dependencies are inferred from the claim text.