Mutant DNase1L3 with improved serum half-life
AssigneeTexas Tech University System
InventorsPeter Keyel, Roger B. Sutton, Jon McCord
Life of this patent
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Abstract
The present invention includes a mutant Dnase1LS having at least about a 95% identity with a nucleic acid sequence encoding the protein of SEQ ID NO: 2-8 for a mutant Dnase1LS comprising at least one mutation for post-translational modification or attachment of a molecule to the mutant Dnase1LS to increase the serum half-life of the mutant Dnase1LS, nucleic acids encoding the same, host cells, and methods of making the mutant Dnase1LS.
Claims13
A mutant Dnase1L3 nucleotide sequence having at least about a 95% identity with a nucleic acid sequence encoding a mutant Dnase1L3 protein having SEQ ID NO:
- 2-8, wherein the mutant Dnase1L3 protein comprises at least one mutation for post-translational modification or attachment of a molecule to the mutant Dnase1L3 to increase a serum half-life of the mutant Dnase1L3, wherein the mutation to Dnase1L3 protein is selected from at least one of:
- an S112C mutation, an S131C mutation, or an S279C mutation;
- or
- at least two mutations selected from S91C, S112C, S131C, and S279C.
A mutant Dnase1L3 protein produced by a method comprising:
- culturing a host cell transformed with an expression vector comprising a DNA sequence comprising a nucleotide sequence encoding the mutant Dnase1L3 protein having at least 95% identity with SEQ IDS NO:
- 2-8;
- expressing the mutant Dnase1L3 protein in the host cell;
- and isolating the mutant Dnase1L3 protein, wherein the mutation to Dnase1L3 protein is selected from at least one of:
- an S112C mutation, an S131C mutation, or an S279C mutation;
- or
- at least two mutations selected from S91C, S112C, S131C, and S279C.
A mutant Dnase1L3 protein comprising at least about a 95% identity with SEQ ID NO:
- 2-8, wherein the Dnase1L3 protein comprises at least one mutation selected from:
- An S112C mutation, an S131C mutation, or an S279C mutation;
- or
- At least two mutations selected from S91C, S112C, S131C and S279C.
Dependencies are inferred from the claim text.