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US 12,577,555 B2Granted
Screening method for telomerase reverse transcriptase (TERT) phosphorylation inhibitors
AssigneeKenkichi Masutomi
InventorsKenkichi Masutomi, Mami Yasukawa
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Abstract
The present invention provides a method for screening for a telomerase reverse transcriptase (TERT) phosphorylation inhibitor using TERT or a fragment thereof, and a TERT kinase.
Claims4
An isolated human telomerase reverse transcriptase (TERT) or a fragment thereof, comprising a part of an amino acid sequence corresponding to the amino acid sequence set forth in SEQ ID NO:
- 17, wherein the TERT and the part comprise an amino acid corresponding to T249 in the amino acid sequence set forth in SEQ ID NO:
- 17,
- (i) wherein the amino acid corresponding to T249 in the TERT or the fragment is replaced with alanine (A), wherein the TERT and the fragment have a telomere elongation enzyme activity, and wherein the TERT or the fragment has a reduced RNA-dependent RNA polymerase (RdRP) activity, compared to a TERT having threonine at position corresponding to T249.
An isolated human telomerase reverse transcriptase (TERT) or a fragment thereof, comprising a part of an amino acid sequence corresponding to the amino acid sequence set forth in SEQ ID NO:
- 17, wherein the TERT and the part comprise an amino acid corresponding to T249 in the amino acid sequence set forth in SEQ ID NO:
- 17,
- (ii) wherein the amino acid corresponding to T249 in the TERT or the fragment is replaced with glutamic acid (E), wherein the TERT and the fragment have a telomere elongation enzyme activity, and wherein the TERT or the fragment has an increased RNA-dependent RNA polymerase (RdRP) activity, compared to a TERT having threonine at position corresponding to T249.
Dependencies are inferred from the claim text.