Systems and methods for generating bacteriophages adapted to infect a target bacterial strain

A method of generating bacteriophages adapted to infect a target bacterial strain, the method comprising:

• exposing host bacteria that are susceptible to an input phage to the input phase in a host chemostat;
• exposing target bacteria that are the same species as the host bacteria, but a different strain which acquired resistance to and are not susceptible to the input phage as input to a target chemostat containing the input phage;
• filtering outflows from the host chemostat and the target chemostat to isolate phage from the host chemostat and the target chemostat;
• combining the outflows into a phage pool;
• introducing the phage pool back into each of the host chemostat and the target chemostat;
• and
• repeating the steps of exposing the host bacteria and target bacteria to the input phage, filtering the outflows, combining the outflows into a phage pool, and introducing the phage pool back into each of the host chemostat and the target chemostat at least until a bacteriophage adapted to infect the target bacteria is generated.