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US 12,577,532 B2Granted
Xeno-free and transgene-free reprograming of mesenchymal stem cells toward neural progenitor cells
AssigneesUniversity of Central Florida Research Foundation, Inc., Institute for Scientific Research and Technology Services (Indicasat)
InventorsKiminobu Sugaya, Luis Sebastian Alexis Valerio
Life of this patent
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Abstract
Disclosed herein are methods and materials for transdifferentiating mesenchymal stem cells into neural progenitor cells.
Claims6
A method of reprogramming mesenchymal stem cells as neural progenitor cells, the method comprising (a) modifying epigenetic status of mesenchymal stem cells by (i) subjecting the mesenchymal stem cells to a first media comprising 5-aza-2′-deoxycytidine;
- (ii) removing the first media from the mesenchymal stem cells, and (iii) subjecting the mesenchymal stem cells from step (ii) to a second media comprising SB431542;
- and (b) contacting the mesenchymal stem cells of step (a) with extracellular vesicles obtained from neural stem cells under conditions to allow the mesenchymal stem cells to convert into neural progenitor cells.
Dependencies are inferred from the claim text.