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US 12,577,529 B2Granted
Methods for producing neural cells
AssigneeQuiver Holdings Inc.
InventorsLuis Williams, Vaibhav Joshi, Graham T. Dempsey
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Abstract
The present invention provides differentiated neural cells and methods for making differentiated neural cells from pluripotent stem cells (PSC) at an industrial scale sufficient for high-throughput assays. The methods of the invention allow billions of PSCs and/or neural cells differentiated from the PSCs to be cryopreserved and expanded at multiple steps.
Claims10
A method for producing neuronal cells, the method comprising:
- obtaining pluripotent stem cells;
- introducing into said cells an inducible genetic element that encodes a pro-neuronal transcription factor;
- inducing expression of the genetic element in said cells, thereby causing differentiation of said cells into neuronal cells;
- cryopreserving the neuronal cells;
- thawing the cryopreserved neuronal cells;
- maintaining the neuronal cells in in a medium supplemented with laminin at a first concentration;
- replacing the medium with a second medium comprising laminin at a second concentration lower than the first concentration;
- and
- replacing the second medium with a third medium comprising laminin at a third non-zero concentration lower than the second concentration.
6 more elements
Dependencies are inferred from the claim text.