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US 12,577,528 B2Granted
Taurine supplemented cell culture medium and methods of use
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsAmy S. Johnson, Meghan E. Casey, Shadia Oshodi, Shawn M. Lawrence
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Abstract
The specification describes a composition comprising an improved eukaryotic cell culture medium, which can be used for the production of a protein of interest. Taurine can be added to the serum-free media or chemically-defined media to increase the production of a protein of interest. Methods for recombinantly expressing high levels of protein using the media compositions are included.
Claims28
A method for cultivating a eukaryotic cell expressing dupilumab, comprising:
- (a) providing a base cell culture medium supplemented with about 0.1 mM to about 10 mM L-taurine;
- (b) propagating or maintaining the eukaryotic cell in the base cell culture medium to form a cell culture;
- and
- (c) producing dupilumab from the cell culture;
- wherein supplementing the base cell culture medium with L-taurine increases the titer of dupilumab by at least 3% when compared to the titer of dupilumab from cells propagated or maintained in a cell culture medium containing less than 0.1 mM L-taurine.
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A method for producing dupilumab comprising the steps of:
- (a) introducing a nucleic acid comprising a nucleotide sequence encoding dupilumab into a cell;
- (b) isolating the cell expressing the dupilumab;
- (c) culturing the isolated cell in a cell culture medium comprising about 0.1 mM to about 10 mM L-taurine, thereby producing a population of cells;
- (d) expressing dupilumab from the population of cells, wherein the dupilumab is secreted into the medium;
- and
- (e) harvesting the dupilumab;
- wherein the addition of about 0.1 mM to about 10 mM L-taurine to the cell culture medium increases the titer of dupilumab by at least 3% when compared to the titer of dupilumab from cells cultured in a cell culture medium containing less than 0.1 mM L-taurine.
4 more elements
Dependencies are inferred from the claim text.