Ultra bright dimeric or polymeric dyes

A method, comprising:

• labeling an analyte molecule, including:
• (a) admixing an intermediary compound with a compound of formula M-L1b-G′, wherein the intermediary compound has the following structure (II), or a stereoisomer, salt or tautomer thereof:
• wherein:
• L1a, L2 and L3 are, at each occurrence, independently an optional alkylene, alkenylene, alkynylene, heteroalkylene, heteroalkenylene, heteroalkynylene or heteroatomic linker;
• L4 is, at each occurrence, independently an alkylene, alkenylene, alkynylene, heteroalkylene, heteroalkenylene or heteroalkynylene linker;
• R1 is, at each occurrence, independently H, alkyl or alkoxy;
• R2 and R3 are each independently H, OH, SH, alkyl, alkoxy, alkylether, —OP(═Ra)(Rb)Rc, Q, a linker comprising a covalent bond to Q, a linker comprising a covalent bond to a solid support or a linker comprising a covalent bond to a further compound of structure (II), wherein:
• Ra is O or S;
• Rb is OH, SH, O−, S−, ORd or SRd;
• Rc is OH, SH, O−, S−, ORd, SRd, alkyl, alkoxy, alkylether, alkoxyalkylether, phosphate, thiophosphate, phosphoalkyl, thiophosphoalkyl, phosphoalkylether or thiophosphoalkylether;
• and Rd is a counter ion, and wherein at least R2 or R3 is Q or a linker comprising a covalent bond to Q;
• R4 is, at each occurrence, independently OH, SH, O−, S−, ORd or SRd;
• R5 is, at each occurrence, independently oxo, thioxo or absent;
• Q is, at each occurrence, independently a moiety comprising a sulfhydryl, disulfide, activated ester, isothiocyanate, azide, alkyne, alkene, diene, dienophile, acid halide, sulfonyl halide, phosphine, α-haloamide, biotin, amino or maleimide functional group, wherein the activated ester is an N-succinimide ester, imidoester or polyflourophenyl ester;
• m is, at each occurrence, independently an integer of zero or greater, wherein at least one occurrence of m is an integer of one or greater;
• and
• n is an integer of one or greater, and
• wherein admixing includes forming at least one covalent bond by reaction of G and G′;
• and
• (b) reacting the product of step (a) with the analyte molecule, including forming a conjugate of the product of step (a) and the analyte molecule, the analyte molecule being covalently attached to an end of the product of step (a);
• wherein:
• G is a moiety including an aldehyde, oxime, hydrazone, alkyne, amine, azide, acylazide, acylhalide, nitrile, nitrone, sulfhydryl, disulfide, sulfonyl halide, isothiocyanate, activated ester, ketone, α, β-unsaturated carbonyl, alkene, maleimide, α-haloimide, epoxide, aziridine, tetrazine, tetrazole, phosphine, biotin, or thiirane functional group, wherein the activated ester is an N-succinimide ester, imidoester or polyflourophenyl ester;
• M is, at each occurrence, independently a moiety comprising two or more carbon-carbon double bonds and at least one degree of conjugation and at least one occurrence of M is a moiety comprising a carbocyclic or a heterocyclic;
• L1b is an optional alkylene or heteroalkylene linker;
• and
• G′ is a reactive group complementary to G.