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US 12,577,327 B2Granted
Mixed binding domains
AssigneeMerus N.V.
InventorCornelis Adriaan De Kruif
Life of this patent
Estimating the term…
Abstract
A binding domain or a multimer or a variant thereof which comprises a variable region encoded by a nucleic acid based on, derived or obtained from an animal phylogenetically distal from a human, which variable region is paired with a human variable region.
Claims23
A phage which comprises in its genome:
- a nucleic acid encoding a bird heavy chain variable region (VH) and
- a nucleic acid encoding a human common light chain variable region (VL).
A method for preparing a phage display library comprising:
- immunizing a bird with an antigen;
- isolating a plurality of nucleic acids encoding heavy chain variable regions from said bird;
- and
- preparing a phage display library using said nucleic acids encoding said heavy chain variable regions and nucleic acids encoding human light chain variable regions that pair with said heavy chain variable regions,
- thereby preparing a phage display library.
2 more elements
A method for the identification of a binding domain or a multimer or a variant thereof that has binding specificity for an antigen, which method comprises:
- immunizing a bird with an antigen;
- isolating a plurality of nucleic acids encoding heavy chain variable regions from said bird;
- preparing a phage display library using said nucleic acids encoding said heavy chain variable regions, wherein said phage display library comprises phages comprising a nucleic acid sequence encoding a human common light chain variable region;
- and
- identifying a phage in the phage display library that binds to the antigen, thereby identifying a binding domain or a multimer or a variant thereof that has binding specificity for the antigen.
2 more elements
Dependencies are inferred from the claim text.