Antibodies having specificity to HER4 and uses thereof
AssigneesInserm (Institut National De La Sante Et De La Recherche Medicale), Institut Regional Du Cancer De Montpellier, Universite De Montpellier
InventorsAndre Pelegrin, Pierre Martineau, Thierry Chardes, Romain Lanotte
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Abstract
The present invention relates to antibodies having specificity to HER4 and uses thereof, which are able to induce biased 4ICD routing/signaling. The inventors have isolated by antibody phage display three fully human anti-HER4 single-chain variable antibody fragment (scFv), selected on human HER4 extracellular domain, referred as C6 mAb, D5 mAb and F4 mAb and one fully human anti-HER4 scFv named H2 mAb, selected on NRG 1β1-stimulated EGFR/HER4 JMaCYT1-transfected NIH3T3 cells. In particular, the present invention relates to an isolated anti-HER4 antibody, wherein said antibody binds to an epitope of the HER4 protein.
Claims16
An isolated anti-HER4 antibody selected from a first, second, third, and fourth mab wherein:
- the first mab, has:
- (a) a heavy chain variable domain which comprises:
- a H-CDR1 having a sequence set forth as SEQ ID NO:
- 2;
- a H-CDR2 having a sequence set forth as SEQ ID NO:
- 3;
- a H-CDR3 having a sequence set forth as SEQ ID NO:
- 4;
- (b) a light chain variable domain which comprises:
- a L-CDR1 having a sequence set forth as SEQ ID NO:
- 6;
- a L-CDR2 having a sequence set forth as SEQ ID NO:
- 7;
- a L-CDR3 having a sequence set forth as SEQ ID NO:
- 8;
- the second mab, has:
- (a) a heavy chain variable domain which comprises:
- a H-CDR1 having a sequence set forth as SEQ ID NO:
- 10;
- a H-CDR2 having a sequence set forth as SEQ ID NO:
- 11;
- a H-CDR3 having a sequence set forth as SEQ ID NO:
- 12;
- (b) a light chain variable domain which comprises:
- a L-CDR1 having a sequence set forth as SEQ ID NO:
- 14;
- a L-CDR2 having a sequence set forth as SEQ ID NO:
- 15;
- a L-CDR3 having a sequence set forth as SEQ ID NO:
- 16;
- the third mab, has:
- (a) a heavy chain variable domain which comprises:
- a H-CDR1 having a sequence set forth as SEQ ID NO:
- 18;
- a H-CDR2 having a sequence set forth as SEQ ID NO:
- 19;
- a H-CDR3 having a sequence set forth as SEQ ID NO:
- 20;
- (b) a light chain variable domain which comprises:
- a L-CDR1 having a sequence set forth as SEQ ID NO:
- 22;
- a L-CDR2 having a sequence set forth as SEQ ID NO:
- 23;
- a L-CDR3 having a sequence set forth as SEQ ID NO:
- 24;
- and the fourth mab, has:
- (a) a heavy chain variable domain which comprises:
- a H-CDR1 having a sequence set forth as SEQ ID NO:
- 26;
- a H-CDR2 having a sequence set forth as SEQ ID NO:
- 27;
- a H-CDR3 having a sequence set forth as SEQ ID NO:
- 28;
- (b) a light chain variable domain which comprises:
- a L-CDR1 having a sequence set forth as SEQ ID NO:
- 30;
- a L-CDR2 having a sequence set forth as SEQ ID NO:
- 31;
- a L-CDR3 having a sequence set forth as SEQ ID NO:
- 32.
Dependencies are inferred from the claim text.