Antibody-mediated transduction of heat shock proteins into living cells
AssigneeThe United States Government as represented by the Department of Veterans Affairs
InventorsRichard H. Weisbart, Robert N. Nishimura, James E. Hansen
Life of this patent
Estimating the term…
Abstract
The invention provides for a fusion protein comprising a 3E10 Fv joined to a Hsp-70, Hsp-27, Hsp-90 or GRP-78 or portion thereof, and optionally, the 3E10 Fv comprising an amino acid sequence AGIH at its amino terminus.
Claims14
A method of treating of subject having a disease or disorder associated with hydrogen peroxide toxicity or ROS toxicity comprising administering to the subject a pharmaceutical composition comprising a fusion protein comprising a 3E10 Fv joined to a HSP-70, a peptide linker comprising an immunoglobulin heavy chain constant domain CH1 or a portion thereof;
- and a swivel sequence, wherein the swivel sequence comprises a peptide sequence located between the 3E10 Fv and the HSP-70 and permits the 3E10 Fv and the HSP-70 to swivel, wherein the swivel sequence consists of the amino acid sequence of residues 375 through 380 of SEQ ID NO:2.
Dependencies are inferred from the claim text.