T cell receptors which recognize mutated EGFR
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsKenichi Hanada, Chihao Zhao, Anna Pasetto, James C. Yang
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Abstract
Disclosed is an isolated or purified T cell receptor (TCR), wherein the TCR has antigenic specificity for a mutated EGFR amino acid sequence with a E746-A750 deletion. Related polypeptides and proteins, as well as related nucleic acids, recombinant expression vectors, host cells, populations of cells, and pharmaceutical compositions are also provided. Also disclosed are methods of detecting the presence of cancer in a mammal and methods of treating or preventing cancer in a mammal.
Claims29
An isolated or purified T cell receptor (TCR) having antigenic specificity for a peptide comprising the mutated epidermal growth factor receptor (EGFR) amino acid sequence of AIKTSPKANKEIL (SEQ ID NO:
- 36) presented by a human leukocyte antigen (HLA) Class II molecule that is a heterodimer of an HLA-DPA1*02:01 chain and an HLA-DPB1*01:01 chain,
- wherein the TCR comprises an α chain comprising an α chain complementarity determining region (CDR) 1 comprising the amino acid sequence of SEQ ID NO:
- 3, an α chain CDR2 comprising the amino acid sequence of SEQ ID NO:
- 4, and an α chain CDR3 comprising the amino acid sequence of SEQ ID NO:
- 5;
- and a β chain comprising a β chain CDR1 comprising the amino acid sequence of SEQ ID NO:
- 6, a β chain CDR2 comprising the amino acid sequence of SEQ ID NO:
- 7, and a β chain CDR3 comprising the amino acid sequence of SEQ ID NO:
- 8.
An isolated or purified protein having antigenic specificity for a peptide comprising the mutated epidermal growth factor receptor (EGFR) amino acid sequence of AIKTSPKANKEIL (SEQ ID NO:
- 36) presented by a human leukocyte antigen (HLA) Class II molecule that is a heterodimer of an HLA-DPA1*02:01 chain and an HLA-DPB1*01:01 chain,
- wherein the protein comprises a first polypeptide chain comprising a T cell receptor (TCR) a chain amino acid sequence comprising an α chain complementarity determining region (CDR) 1 comprising the amino acid sequence of SEQ ID NO:
- 3, an α chain CDR2 comprising the amino acid sequence of SEQ ID NO:
- 4, and an α chain CDR3 comprising the amino acid sequence of SEQ ID NO:
- 5;
- and a second polypeptide chain comprising a TCR β chain amino acid sequence comprising a β chain CDR1 comprising the amino acid sequence of SEQ ID NO:
- 6, a β chain CDR2 comprising the amino acid sequence of SEQ ID NO:
- 7, and a β chain CDR3 comprising the amino acid sequence of SEQ ID NO:
- 8.
Dependencies are inferred from the claim text.