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US 12,577,280 B2Granted
Glycosylated comp pilin variants, methods of making and uses thereof
AssigneeVaxnewmo LLC
InventorsChristian Harding, Mario Feldman
Life of this patent
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Abstract
Provided herein are glycosylated ComP proteins, fragments and fusion proteins thereof, and methods of making, for example, for use in the production of conjugate vaccines. Also provided herein are conjugate vaccines against diseases including bacterial diseases.
Claims21
A ComP glycosylation tag comprising an isolated fragment of a ComP protein, wherein the fragment comprises a serine residue corresponding to the conserved serine residue at position 82 of SEQ ID NO:
- 2 (ComP110264:
- ENV58402.1),
- wherein the glycosylation tag is not more than 50 amino acids in length.
A ComP glycosylation tag comprising the amino acid consensus sequence of:
- X14GVX17X18IX20X21X22ASX25X26TX28NVX31X32AX34 (SEQ ID NO:
- 38),
- wherein:
- X14 is V, T, or A;
- X17 is Q, T, or E;
- X18 is E, Q, or T;
- X20 is S, N, A, or G;
- X21 is S or no amino acid;
- X22 is G or no amino acid;
- X25 is N, S, or A;
- X26 is A, S, or K;
- X28 is T, S, or K;
- X31 is A or E;
- X32 is T or S;
- or
- X34 is T, Q, or A,
- or a fragment of at least 19 consecutive amino acids thereof, wherein said glycosylation tag fragment comprises the serine residue corresponding to the conserved serine residue at position 24 of SEQ ID NO:
- 38, or a variant thereof having one, two, three, four, five, six, or seven amino acid substitutions, additions, and/or deletions, wherein the variant maintains the serine residue corresponding to the conserved serine residue at position 24 of SEQ ID NO:
- 38,
- wherein the glycosylation tag is not more than 50 amino acids in length.
17 more elements
Dependencies are inferred from the claim text.