Hydrophobic interaction chromatography carrier and protein purification method
AssigneeJnc Corporation
InventorsKojiro Sota, Yoshihiro Matsumoto
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Abstract
A chromatography carrier capable of removing an antibody dimer from a solution containing an antibody monomer. The chromatography carrier includes a base carrier containing porous particles and a hydrophobic ligand bound to the base carrier, and has an electric conductivity of 34 mS/cm or less measured by a gradient elution test. The porous particles preferably have an average particle diameter of 66 to 150 μm, and the hydrophobic ligand preferably has at least one selected from a group consisting of phenyl, n-butyl, n-hexyl, n-octyl, and n-octadecyl.
Claims6
A method for purifying an antibody of which a dimer is removed, the method comprising:
- (1) selecting a chromatography carrier having a base carrier, wherein the base carrier comprises porous particles and a hydrophobic ligand bound to the base carrier, wherein the selecting comprises:
- (a) performing a gradient elution test, comprising:
- flowing a lysozyme solution comprising a buffer through the chromatography carrier and allowing the lysozyme solution to elute from the chromatography carrier, and
- measuring electric conductivity of the buffer of the lysozyme solution at the top of the elution peak of the lysozyme solution;
- and
- (b) selecting the chromatography carrier when the buffer of the lysozyme solution has an electric conductivity of 34 mS/cm or less at the top of the elution peak of the lysozyme solution;
- and
- (2) bringing the selected chromatography carrier into contact with an antibody solution comprising a dimer of an antibody;
- and
- (3) recovering a solution from the selected chromatography carrier, wherein the dimer has been removed from the recovered solution.
Dependencies are inferred from the claim text.