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US 12,576,404 B2Granted
Disposable reagent scaffold for biochemical process integration
AssigneeThe Johns Hopkins University
InventorsTza-Huei Wang, Dong Jin Shin, Alexander Y. Trick
Life of this patent
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Abstract
Devices for assaying a biomolecule from a sample, including: a cartridge assembly structured to accept and secure a magnetofluidic cartridge to be used for the assaying, and a magnetic particle manipulation assembly arranged proximate the cartridge assembly, the magnetic particle manipulation assembly having a pair of magnets arranged to be on opposing sides of said magnetofluidic cartridge and which are substantially aligned along a line that will be transverse to the magnetofluidic cartridge such that the line can be aligned with a well in the magnetofluidic cartridge, and methods of using and assembling such devices.
Claims24
A magnetofluidic device for assaying a biomolecule from a sample, comprising:
- a magnetofluidic cartridge having a plurality of wells configured for said assaying, at least one well of the plurality of wells comprising a plurality of magnetic particles;
- a cartridge assembly securing the magnetofluidic cartridge;
- a magnetic particle manipulation assembly arranged proximate said cartridge assembly, said magnetic particle manipulation assembly comprising:
- a first magnet arranged at a first side of the magnetofluidic cartridge and a second magnet arranged at a second side opposite the first side of the magnetofluidic cartridge such that the first magnet and the second magnet are on opposing sides of said magnetofluidic cartridge, the first magnet and the second magnet are substantially aligned along a line that is transverse to said magnetofluidic cartridge such that said line is alignable with a first well of the plurality of wells in said magnetofluidic cartridge, and a dual-axis actuator configured to drive the first magnet and the second magnet;
- and
- a temperature control assembly arranged proximate said cartridge assembly, said temperature control assembly comprising a heat exchange portion structured and arranged to be in thermal connection with a second well of the plurality of wells,
- wherein at least one of the first magnet and the second magnet is movable, by the dual-axis actuator, in a first degree of freedom along said line relative to said magnetofluidic cartridge such that the plurality of magnetic particles when contained within said first well will be drawn out of said first well during operation along the first degree of freedom, and
- wherein the first magnet and the second magnet are movable, by the dual-axis actuator, along a second degree of freedom parallel to a surface of the magnetofluidic cartridge to translate said plurality of magnetic particles along said surface of the magnetofluidic cartridge such that said line is alignable with said second well, and
- wherein at least one of the first magnet and the second magnet are movable, by the dual-axis actuator, in said first degree of freedom along said line relative to said magnetofluidic cartridge such that the plurality of magnetic particles will be drawn into said second well in said magnetofluidic cartridge.
6 more elements
A method of detecting a biomolecule in a sample using a magnetofluidic device for assaying a biomolecule from a sample, comprising:
- a magnetofluidic cartridge having a plurality of wells configured for said assaying, at least one well of the plurality of wells comprising a plurality of magnetic particles;
- a cartridge assembly structured to accept and secure the magnetofluidic cartridge;
- and a magnetic particle manipulation assembly arranged proximate said cartridge assembly, said magnetic particle manipulation assembly comprising a first magnet arranged at a first side the magnetofluidic cartridge and a second magnet arranged at a second side opposite the first side of the magnetofluidic cartridge such that the first magnet and the second magnet are on opposing sides of said magnetofluidic cartridge, the first magnet and the second magnet are substantially aligned along a line that is transverse to said magnetofluidic cartridge such that said line is aligned with a first well of the plurality of wells in said magnetofluidic cartridge, and a dual-axis actuator configured to drive the first magnet and the second magnet;
- and a temperature control assembly arranged proximate said cartridge assembly, said temperature control assembly comprising a heat exchange portion structured and arranged to be in thermal connection with the at least one well of the plurality of wells, the method comprising:
- loading said sample into a sample well of a magnetofluidic cartridge so as to contact said biomolecule with a magnetic particle such that the biomolecule binds to the magnetic particle;
- moving, by the dual-axis actuator, the first magnet in a first degree of freedom along said line relative to said magnetofluidic cartridge so as to move the magnetic particle bound to the biomolecule along the first degree of freedom to draw the magnetic particle bound to the biomolecule out of the sample well and into a spacer layer of said magnetofluidic cartridge;
- moving, by the dual-axis actuator, the first magnet along a second degree of freedom to translate the magnetic particle bound to the biomolecule along the second degree of freedom to translate the magnetic particle bound to the biomolecule within the spacer layer to a position at a detection well of said magnetofluidic cartridge;
- drawing, using the second magnet, the magnetic particle bound to the biomolecule along the first degree of freedom out of the spacer layer and into the detection well;
- heating the biomolecule such that amplification of the biomolecule occurs;
- and
- detecting the biomolecule.
8 more elements
Dependencies are inferred from the claim text.