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US 12,576,400 B2Granted
Methods for incubating and analyzing a cell in a compartment of a fluidic device
AssigneeCellanome, Inc.
InventorsTarun Kumar Khurana, Ali Agah, Yir-Shyuan Wu, Filiz Gorpe Yasar, Vernon A. Norviel
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Abstract
Described herein are systems and methods for analyzing biological samples. The methods can include polymerizing a polymer precursor in the fluidic device, thereby forming a compartment comprising a polymer matrix wall. The compartment may enclose a cell within the fluidic device. The method may further include incubating the cell while the cell is within the compartment. The method may also include performing an assay within the compartment.
Claims27
A method for analyzing a cell, comprising:
- a) providing energy to a fluidic device comprising a fluid to polymerize a polymer precursor in the fluidic device, thereby forming a compartment comprising a polymer matrix wall within the fluid, wherein the fluid comprises a plurality of cells, wherein the compartment encloses a cell of the plurality of cells, and wherein the compartment physically separates the cell from an additional cell of the plurality of cells;
- b) incubating the cell for at least about 1 minute, wherein the cell is inside of the compartment during the incubating;
- and
- c) performing an assay within the compartment, thereby analyzing the cell, wherein the assay comprises:
- lysing the cell, thereby releasing a nucleic acid from the cell;
- capturing the nucleic acid using a capture element, wherein the capture element is inside of the compartment;
- and
- reverse transcribing the nucleic acid to form a cDNA molecule.
5 more elements
A method for analyzing a cell, comprising:
- a) providing energy to a fluidic device comprising a fluid to polymerize a polymer precursor in the fluidic device, thereby forming a compartment comprising a polymer matrix wall within the fluid, wherein the fluid comprises a plurality of cells, wherein the compartment encloses a cell of the plurality of cells, and wherein the compartment physically separates the cell from an additional cell of the plurality of cells;
- b) incubating the cell for at least about 1 minute, wherein the cell is inside of the compartment during the incubating;
- and
- c) performing an assay within the compartment, thereby analyzing the cell, wherein the assay comprises detecting a protein secreted by the cell.
1 more element
A method for analyzing a cell, comprising:
- a) providing energy to a fluidic device comprising a fluid to polymerize a polymer precursor in the fluidic device, thereby forming a compartment comprising a polymer matrix wall within the fluid, wherein the fluid comprises a plurality of cells, wherein the compartment encloses a cell of the plurality of cells with antibodies specific for surface proteins, and wherein the compartment physically separates the cell from an additional cell of the plurality of cells;
- b) incubating the cell for at least about 1 minute, wherein the cell is inside of the compartment during the incubating;
- and
- c) performing an assay within the compartment, thereby analyzing the cell, wherein the assay comprises determining a relative expression of the surface proteins on the cell.
1 more element
A method for analyzing a cell, comprising:
- a) providing energy to a fluidic device comprising a fluid to polymerize a polymer precursor in the fluidic device, thereby forming a compartment comprising a polymer matrix wall within the fluid, wherein the fluid comprises a plurality of cells, wherein the compartment encloses a cell of the plurality of cells, and wherein the compartment physically separates the cell from an additional cell of the plurality of cells;
- b) incubating the cell for at least about 1 minute, wherein the cell is inside of the compartment during the incubating;
- and
- c) performing an assay within the compartment, wherein the assay comprises detecting a nucleic acid from the cell.
1 more element
Dependencies are inferred from the claim text.