US 12,576,345 B2Granted

Chromatography column packing medium recovery

AssigneeRepligen Corporation

InventorsTravis R. Ward, James Ronald Peyser, Dana Pentia, Evan Frankel

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Abstract

Systems include a chromatography column tube having an inlet and outlet and port assembly arranged in a wall of the column tube between flow distributors that together form a chamber within the tube that is filled with packing medium are described. The port assembly facilitates the removal of resin from the pre-packed column and the port assembly does not affect fluid flow in the normal use of the column for chromatographic separation or ability to maintain sanitary conditions within the column. Also described are methods that include attaching tubing to a pump and to a column inlet and a column outlet, opening the port assembly, attaching tubing to the port assembly and to a second reservoir, and pumping aqueous solution from a reservoir into the chamber and out through the port assembly into the second reservoir, thereby removing packing medium from the column along with the flowing aqueous solution.

Claims7

Dependent claims

A chromatography column comprising:

a column tube comprising a cylindrical wall, the column tube having a first end and a second end;
a first flow distributor arranged within the first end of the column tube and a second flow distributor arranged within the second end of the column tube;
an opening in the cylindrical wall of the column tube between locations where the first flow distributor and the second flow distributor are secured during use;
and
a recovery port assembly secured within the opening in the cylindrical wall of the column tube, wherein the recovery port assembly is configured to permit removal of a fluid from within the column tube in a chamber formed between the first and second flow distributors,
the recovery port assembly comprising:
a port body machined from a rigid plastic extending through the opening in the cylindrical wall of the column tube, wherein the port body comprises a beveled flange disposed within the chamber;
a plug comprising an external end extending outside the port body configured to facilitate removal of the plug from the port body;
and
a clamp configured to secure the plug within the port body.

7 more elements

Dependencies are inferred from the claim text.

Facts

Application: 16/308,629Filed 12 Jun 2017 · us provisional application
Priority: 10 Jun 2016
Published: US 2019/0270034 A15 Sep 2019
Granted: 17 Mar 2026
Term ends: Not estimatedThe source records don’t support an estimate.

Classification7 codes

B01D 15/22
Characterised by constructional or operational features: relating to the construction of the column
B01D 15/20
Characterised by constructional or operational features: relating to the conditioning of the sorbent material
B01D 15/206
Relating to the conditioning of the sorbent material: packing or coating
G01N 30/56
Conditioning of the sorbent material or stationary liquid: packing methods or coating methods