Temperature-assisted on-column focusing
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorsStephen Weber, Stephen Robert Groskreutz, Anthony Robert Horner, Rachael-Anh Elizabeth Wilson
Life of this patent
Estimating the term…
Abstract
A method comprising: introducing a sample volume into an inlet end of a liquid chromatography column, wherein the liquid chromatography column includes a focusing segment proximal to the inlet end of the liquid chromatography column and a separation segment proximal to an elute outlet of the liquid chromatography column; maintaining only the focusing segment at a first temperature as the sample is introduced into the focusing segment; and subsequently heating the focusing segment to a second temperature that is higher than the first temperature after the entire sample volume has been introduced into the focusing segment.
Claims28
A method comprising:
- introducing a sample having a predetermined volume into an inlet end of a liquid chromatography column, wherein the liquid chromatography column includes a first focusing segment proximal to the inlet end of the liquid chromatography column, a separation segment proximal to an elute outlet of the liquid chromatography column, and at least one second focusing segment located between the first focusing segment and the separation segment;
- maintaining the first focusing segment and the at least one second focusing segment at a first temperature as the sample is introduced into the first focusing segment;
- subsequently heating the first focusing segment to a second temperature that is higher than the first temperature while maintaining the at least one second focusing segment at the first temperature, wherein the subsequent heating of the first focusing segment is initiated after all of the predetermined sample volume has passed into the first focusing segment;
- subsequently heating the at least one second focusing segment to a third temperature that is higher than the first temperature;
- and
- heating the separation segment to the second temperature or the third temperature.
Dependencies are inferred from the claim text.