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US 12,576,168 B2Granted
Peptide PET/SPECT probes specific to oncoproteins in tumor extracellular matrix
AssigneeCase Western Reserve University
InventorsZheng-Rong Lu, Songqi Gao
Life of this patent
Estimating the term…
Abstract
A PET/SPECT probe includes the following formula: P-L-C wherein P is a EDB-FN targeting peptide, C is a PET/SPECT contrast agent; and L is an optional linker that covalently links the peptide to the contrast agent.
Claims13
A PET/SPECT probe comprising formula:
- P-L-C
- wherein P is a peptide that binds to extradomain-B fibronectin (EDB-FN) or extradomain-A fibronectin (EDA-FN) and that includes an amino acid sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 19, SEQ ID NO:
- 20, SEQ ID NO:
- 21, SEQ ID NO:
- 22, SEQ ID NO:
- 23, SEQ ID NO:
- 24, and retro-inverso amino acid sequences thereof;
- C is a PET/SPECT contrast agent;
- and L is an optional linker that covalently links the peptide to the PET/SPECT contrast agent,
- wherein the contrast agent is a metal chelating agent comprising at least one of diethylenetriaminepentaacetate (DTPA), 1,4,7,10-tetraazadodecanetetraacetate (DOTA), 1,4,7,10-tetraazadodecane-1,4,7-triacetate (DO3A), ethylenediaminetetraacetate (EDTA), 1,4,7,10-tetraazacyclotridecanetetraacetic acid (TRITA), 1,4,8,11-tetraazacyclotetradecane-1,4,8,11-tetraacetic acid (TETA), 1,4,7,10-tetraazadodecanetetramethylacetate (DOTMA), 1,4,7,10-tetraazadodecane-1,4,7-trimethylacetate (DO3MA), N,N′,N″,N″′-tetraphosphonatomethyl-1,4,7,10-tetraazacyclododecane (DOTP), 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetrakis(methylene methylphosphonic acid) (DOTMP), 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetrakis(methylene phenylphosphonic acid) (DOTPP), N,N′-ethylenedi-L-cysteine, and salts or esters thereof.
17 more elements
A method of detecting cancer cells in tissue of a subject comprising:
- contacting the tissue of the subject with a PET/SPECT probe, the PET/SPECT probe comprising the following formula:
- P-L-C
- wherein P is a peptide that binds to extradomain-B fibronectin (EDB-FN) or extradomain-A fibronectin (EDA-FN) and that includes an amino acid sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 19, SEQ ID NO:
- 20, SEQ ID NO:
- 21, SEQ ID NO:
- 22, SEQ ID NO:
- 23, SEQ ID NO:
- 24, and retro-inverso amino acid sequences thereof;
- C is a PET/SPECT contrast agent;
- and L is an optional linker that covalently links the peptide to the PET/SPECT contrast agent,
- wherein the contrast agent is a metal chelating agent comprising at least one of diethylenetriaminepentaacetate (DTPA), 1,4,7,10-tetraazadodecanetetraacetate (DOTA), 1,4,7,10-tetraazadodecane-1,4,7-triacetate (DO3A), ethylenediaminetetraacetate (EDTA), 1,4,7,10-tetraazacyclotridecanetetraacetic acid (TRITA), 1,4,8,11-tetraazacyclotetradecane-1,4,8,11-tetraacetic acid (TETA), 1,4,7,10-tetraazadodecanetetramethylacetate (DOTMA), 1,4,7,10-tetraazadodecane-1,4,7-trimethylacetate (DO3MA), N,N′,N″,N″′-tetraphosphonatomethyl-1,4,7,10-tetraazacyclododecane (DOTP), 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetrakis(methylene methylphosphonic acid) (DOTMP), 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetrakis(methylene phenylphosphonic acid) (DOTPP), N,N′-ethylenedi-L-cysteine, and salts or esters thereof;
- and
- detecting a location and/or distribution of cancer cells in the tissue by detecting the PET/SPECT probe in the tissue of the subject.
20 more elements
Dependencies are inferred from the claim text.