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US 12,576,162 B2Granted
Cell penetrating peptides and uses thereof
AssigneeIucf-Hyu (Industry-University Cooperation Foundation Hanyang University)
InventorsJe-Min Choi, Ja-Hyun Koo
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Abstract
A novel cytokine-derived cell penetrating peptide and use thereof are provided. The cell penetrating peptide has the ability to effectively deliver a biologically active substance into phagocytes, particularly macrophages, both in vitro and in vivo. The cell penetrating peptide can deliver a biologically active substance into macrophages with high efficiency compared to TAT peptide and dNP2 peptide that are commercially available as cell penetrating peptides.
Claims14
A fusion product consisting of:
- a cell penetrating peptide,
- a biologically active substance, and
- a fusing moiety linking the cell penetrating peptide and the biologically active substance,
- wherein the cell penetrating peptide consists of an amino acid sequence represented by the following formula 1:
- (SEQ ID NO:
- 77)
- (Xaa1)a-Arg-Xaa2-Arg-Leu-Arg-Arg-Xaa3-His-(Xaa4)b
- (1)
- wherein each Xaa1 is independently an amino acid selected from the group consisting of leucine, arginine, and lysine, Xaa2 is an amino acid selected from the group consisting of alanine, glycine, proline, leucine, and methionine, Xaa3 is an amino acid selected from the group consisting of alanine, and cysteine, each Xaa4 is arginine, a is an integer from 0 to 2, and b is an integer from 0 to 2, wherein the cell penetrating peptide is composed of 8 to 12 amino acid residues, and
- the biologically active substance is an LRR (leucine rich repeat) domain derived from NLRX1 protein, consisting of the sequence set forth in SEQ ID NO:
- 25.
8 more elements
Dependencies are inferred from the claim text.