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US 12,576,146 B2Granted
Method for producing reassortant influenza viruses
AssigneeSeqirus UK Limited
InventorsSteven Rockman, Chi Ong, Erin Verity
Life of this patent
Estimating the term…
Abstract
A method for producing reassortant influenza viruses is provided. Also provided are reassortant influenza viruses produced according to the method, as well as vaccines based on said reassortant influenza viruses.
Claims24
A method for generating a reassortant influenza virus, comprising the steps of:
- (i) contacting a culture host with a parent influenza virus strain comprising a first hemagglutinin (HA) gene and a first neuraminidase (NA) gene;
- (ii) introducing into the culture host one or more expression construct(s) comprising one or more influenza genes, wherein said influenza genes comprise a second HA gene or a second NA gene;
- (iii) culturing the culture host to produce reassortant viruses;
- and
- (iv) selecting for the reassortant virus that comprises the second HA gene or the second NA gene,
- wherein:
- step (iv) comprises negative selection against the first HA or first NA, wherein the negative selection comprises contacting the culture host or the reassortant influenza viruses that have been separated from the culture host with one or more antibodies which are specific for the first HA or with one or more antibodies which are specific for the first NA.
4 more elements
A method for generating a reassortant influenza virus, comprising the steps of:
- (i) contacting a culture host with a parent influenza virus strain comprising a first hemagglutinin (HA) gene and a first neuraminidase (NA) gene;
- (ii) introducing into the culture host one or more expression construct(s) comprising one or more influenza genes, wherein said influenza genes comprise a second HA gene or a second NA gene;
- (iii) culturing the culture host to produce reassortant viruses;
- and
- (iv) selecting for the reassortant virus that comprises the second HA gene or the second NA gene,
- wherein step (iv) comprises negative selection against the first HA or first NA, wherein the negative selection comprises contacting the culture host or the reassortant influenza viruses that have been separated from the culture host with one or more antibodies which are specific for the first HA or with one or more antibodies which are specific for the first NA, and
- wherein the method comprises a step of pre-passaging an influenza virus strain in cell culture or eggs to produce the parent influenza strain.
4 more elements
Dependencies are inferred from the claim text.