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US 12,576,142 B2Granted
Universal donor cells and related methods
AssigneeViaCyte, Inc.
InventorsAnindita Bhoumik, Alan D. Agulnick, Kevin Allen D'Amour
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Abstract
Disclosed herein are universal donor stem cells and cells derived therefrom and related methods of their use and production. The universal donor stem cells disclosed herein are useful for overcoming allogeneic immune rejection in cell-based transplantation therapies. In certain embodiments, the universal donor cells disclosed herein are pancreatic endoderm cells that do not express one or more MHC-Class I cell-surface proteins and whose expression of at least one NK activating ligand is disrupted or inhibited.
Claims13
A method of producing insulin in a human subject with type I diabetes, comprising:
- transplanting a cell encapsulation device comprising pancreatic endoderm cells (PEC) that are CHGA−, NKX6.1+, PDX1+ into a location in the human subject,
- wherein the function of at least one major histocompatibility complex (MHC)-Class I gene and at least one natural killer (NK) cell activating ligand is disrupted or inhibited in the PEC, resulting in reduced binding of the NK activating ligand to a NK activating receptor in PEC, and
- wherein the PEC mature in the human subject to produce insulin in response to glucose stimulation.
Dependencies are inferred from the claim text.