Genetically engineered microorganisms and methods of use

A genetically engineered microorganism capable of producing functional microcin 147 that can inhibit the growth of bacteria, wherein the microorganism comprises

• a first microcin operon,
• a first controllable promoter for the first microcin operon,
• a second microcin operon, and
• a second controllable promoter for the second microcin operon;
• wherein the first microcin operon comprises microcin genes mciI, mciA, mchC, mchD, mchE, and mchF, but not mchS1 or mchS4,
• wherein the first controllable promoter controls a level of expression of at least one of the microcin genes, thereby controlling an amount of microcin produced by the genetically engineered microorganism,
• wherein the second microcin operon comprises microcin gene mchA, and wherein the second controllable promoter controls a level of expression of the mchA gene, thereby controlling an amount of microcin produced by the genetically engineered microorganism,
• wherein either or both of the first microcin operon and the first controllable promoter are heterologous to the microorganism, and
• wherein the functional microcin I47 inhibits one or more of Klebsiella, Salmonella, Shigella, and Escherichia.