Application of BMSC-exos in treating PD

A method for preparing medicines for treating Parkinson's disease (PD) using human Bone Marrow Mesenchyml Stem Cell Exosomes (BMSC-Exos), comprising the following steps:

• stimulating the human BMSCs with a culture solution and generating the human BMSC-Exos;
• and
• extracting the human BMSC-Exos from the culture solution after passage, the culture solution of the human BMSCs is an Alpha Modified Eagle Medium (α-MEM) culture solution containing Fetal Bovine Serum (FBS) and Penicillin-Streptomycin Solution (PS);
• wherein, during culture the human BMSCs are digested with trypsin when the cell density reaches 80%, and are added with a complete medium of the α-MEM to terminate the digestion when it is observed that the cells become round and intercellular spaces become larger under a microscope;
• a supernatant is discarded after centrifugation, and the passage ratio is determined based on the number of cells;
• the cells are firstly passaged to form a P1 generation, the medium is completely replaced every 2 days, the cells are passaged to form a P2 generation after 4-6 days and then are digested again and passaged to form a P3 generation;
• and then, the P3 generation is used for extracting human BMSC-Exos;
• wherein a method for extracting the human BMSC-Exos comprises the following steps:
• collecting a supernatant of the human BMSCs, adding a buffer XBP with the same volume as the supernatant of the human BMSCs to be mixed uniformly, and filtering and collecting human BMSC-Exos with a membrane affinity spin column;
• wherein a method for collecting the supernatant of human BMSCs comprises the following steps:
• discarding the original culture medium when the P3 generation of human BMSCs grows to have a density of 80%;
• replacing with a serum-free α-MEM culture medium after washing twice with PBS, collecting the culture medium after continuing to culture for 48 h;
• and filtering to obtain supernatant after removing cell debris through centrifugation.