Devices, systems, and methods for tumor visualization and removal

An imaging device, comprising:

• a body having a first end portion configured to be held in a user's hand and a second end portion configured to direct light onto a surgical margin;
• at least one excitation light source configured to excite autofluorescence emissions of tissue cells and fluorescence emissions of induced porphyrins in tissue cells of the surgical margin;
• a white light source configured to illuminate the surgical margin during white light imaging of the surgical margin;
• an imaging sensor;
• a first optical filter configured to filter optical signals emitted by the surgical margin responsive to illumination with excitation light and permit passage of autofluorescence emissions of tissue cells and fluorescence emissions of the induced porphyrins in tissue cells to the imaging sensor;
• and
• a second optical filter configured to filter optical signals emitted by the surgical margin responsive to illumination with white light and permit passage of white light emissions of tissues in the surgical margin to the imaging sensor,
• wherein the excitation light source is adjacent the first optical filter and the white light source is adjacent the second optical filter.

An imaging device, comprising:

• a body having a first end portion configured to be held in a user's hand and a second end portion configured to direct light onto a surgical margin;
• a first excitation light source configured to emit excitation light having a first wavelength;
• a second excitation light source configured to emit excitation light having a second wavelength;
• an imaging sensor configured to detect emissions of the surgical margin;
• a first optical filter configured to filter optical signals emitted by the surgical margin responsive to illumination of the surgical margin with the first excitation light, the first optical filter configured to permit optical signals having a wavelength corresponding to a first characteristic of the surgical margin to pass through the first optical filter to the imaging sensor;
• and
• a second optical filter configured to filter optical signals emitted by the surgical margin responsive to illumination of the surgical margin with the first excitation light, the second optical filter configured to permit optical signals having a wavelength corresponding to a second characteristic of the surgical margin, different from the first characteristic, to pass through the second optical filter to the imaging sensor, and
• a lens element,
• wherein the lens element, the imaging sensor, the first optical filter, and the second optical filter are located at a distal end portion of the body of the imaging device, and
• wherein the lens element directly covers the imaging sensor, the first optical filter, and the second optical filter at the second end portion of the body of the imaging device.

A method of imaging tissue at a surgical margin or a surgical cavity, comprising:

• illuminating the tissue at the surgical margin or the surgical cavity with a first excitation light source configured to emit excitation light having a first wavelength;
• receiving optical signals emitted by the tissue at the surgical margin or the surgical cavity through a first optical filter in an imaging device;
• illuminating the tissue at the surgical margin or the surgical cavity with a second excitation light source configured to emit excitation light having a second wavelength;
• and
• receiving optical signals emitted by the tissue at the surgical margin or the surgical cavity through a second optical filter in the imaging device,
• wherein the first excitation light source is adjacent the first optical filter and the second excitation light source is adjacent the second optical filter.

A method of visualizing breast cancer cells, comprising:

• administering one or more imaging agents to a patient, wherein at least one of the imaging agents is configured to induce formation of at least one protoporphyrin in breast cancer cells;
• subsequent to administration of the one or more imaging agents, illuminating breast tissue of the patient with a first excitation light source configured to cause protoporphyrin to fluoresce;
• receiving, by an imaging sensor, optical signals responsive to illumination with the first excitation light and emitted by cells within the breast tissue containing protoporphyrin through a first optical filter in an imaging device;
• illuminating the breast tissue with a white light source;
• and
• receiving, by the imaging sensor, optical signals responsive to illumination with the white light and emitted by the breast tissue through a second optical filter in the imaging device,
• wherein the imaging sensor, the first optical filter, and the second optical filter are located at a distal end portion of a body of the imaging device.

A portable, handheld imaging device, comprising:

• a body portion having a first end portion configured to be held in a user's hand and a second end portion configured to direct light into a surgical margin, the second end portion having a distal tip comprising:
• at least one excitation light source configured to excite autofluorescence emissions of tissue cells and fluorescence emissions of induced porphyrins in tissue cells of the surgical margin,
• a white light source configured to illuminate the surgical margin during white light imaging of the surgical margin,
• an infrared light source,
• a first imaging sensor configured to support a first imaging mode,
• a first optical filter configured to filter optical signals emitted by the surgical margin responsive to illumination with excitation light and permit passage of autofluorescence emissions of tissue cells and fluorescence emissions of the induced porphyrins in tissue cells to the first imaging device,
• a second imaging sensor configured to support a second imaging mode, and
• a second optical filter configured to filter optical signals emitted by the surgical margin responsive to illumination with white light and permit passage of white light emissions of tissues in the surgical margin to the second imaging device,
• wherein the first imaging sensor, the first optical filter, the second imaging sensor, and the second optical filter are located at a distal end portion of the body of the imaging device.

A portable, handheld imaging device, comprising:

• a body having a first body portion configured to be held in a user's hand and a second body portion having a first end connected to the first body portion and a second free end extending from the body portion, a length of the second body portion extending between the first and second ends along a longitudinal axis of the second body portion, wherein the second free end forms an angle with respect to the longitudinal axis of the second body portion and is configured to direct light onto a surgical margin, the second free end comprising:
• a first optical sensor positioned behind a first optical filter and a plurality of excitation light sources positioned around the first optical filter, the first optical filter being configured to filter optical signals emitted by the surgical margin responsive to illumination with excitation light emitted by the plurality of excitation light sources and to permit passage of autofluorescence emissions of tissue cells and fluorescence emissions of induced porphyrins in cancerous tissue cells to the first optical sensor, and
• a second optical sensor positioned behind a second optical filter, one or more white light sources and one or more infrared light sources being positioned around the second optical filter, the second optical filter being configured to filter optical signals reflected by the surgical margin responsive to illumination with white light and to permit passage of the reflected white light from tissues in the surgical margin to the second optical filter, the second optical filter being positioned adjacent to the first optical filter;
• a controller configured to switch the portable, handheld imaging device between a fluorescence imaging mode, a white light imaging mode, and an infrared imaging mode;
• a processor configured to receive fluorescence data, white light data, and infrared data from the first and second optical sensors and further configured to output one or more images comprising one or more of fluorescence data, white light data, and infrared data.