Bimodal method for detection and measurement of macular pigments in retina tissue

A computing apparatus comprising:

• one or more processors;
• and
• a memory storing instructions that, when executed by the one or more processors, configure the computing apparatus to:
• use a first spectroscopic technique at a first wavelength to measure a first fluorescence value from a fluorescing pigment in a first region of an eye and measure a second fluorescence value from the fluorescing pigment in a second region of the eye, the first region of the eye includes a greater density of one or more macular pigments than the second region of the eye;
• use a second spectroscopic technique at a second wavelength of light that is incident on the eye to measure absorptions in the first region of the eye and the second region of the eye due to other pigments than the fluorescing pigment and the one or more macular pigments, thereby providing absorption values;
• and
• determine an amount of the one or more macular pigments in the first region of the eye using the first fluorescence value, the second fluorescence value, and the absorption values.

A non-transitory computer-readable storage medium, the computer-readable storage medium including instructions that when executed by a computer, cause the computer to:

• using a first spectroscopic technique at a first wavelength to measure a first fluorescence value from a fluorescing pigment in a first region of an eye and measure a second fluorescence value from the fluorescing pigment in a second region of the eye, the first region of the eye includes a greater density of one or more macular pigments than the second region of the eye;
• using a second spectroscopic technique at a second wavelength of light that is incident on the eye to measure absorptions in the first region of the eye and the second region of the eye due to other pigments than the fluorescing pigment and the one or more macular pigments, thereby providing absorption values;
• and
• determine an amount of the one or more macular pigments in the first region of the eye using the first fluorescence value, the second fluorescence value, and the absorption values.