Methods of using a colloidal silver-based composition in reducing or preventing microbial contamination in plants or explants in tissue culture processes
AssigneeClearLeaf Inc.
InventorsAgustin Buchert, Robin A. Wiscovitch, Lawrence Pratt
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Abstract
This disclosure provides methods of using an eco-friendly colloidal silver-based composition in preventing and reducing contamination caused by microorganisms in the plant tissue culture processes. The composition comprises colloidal silver, a copolymer, surfactant, a base, and water. The methods allow cultivating plants or explants in a plant tissue culture medium under non-aseptic conditions and thus obviate the need of the cumbersome and laborious procedures to sterilize the culture medium and the culture vessel in order to create aseptic conditions for plant or explant cultivation.
Claims19
A method for reducing or preventing microbial contamination in plants or explants in a tissue culture process, comprising cultivating a plant or explant in a plant tissue culture medium formed from a colloidal silver-based composition comprising:
- 0.1-20% (w/v) colloidal silver,
- 1.75-4.38% (w/v) methylvinyl ether copolymer,
- 0.02-0.099% (w/v) polyoxyethylene octylphenyl ether,
- 0.02-0.04% (w/v) sodium hydroxide, and
- 89-95% (w/v) water.
Dependencies are inferred from the claim text.