Back to results
US 12,571,805 B2Granted
Methods and compositions for determining OxPL-associated diseases and disorders
AssigneeThe Regents of the University of California
InventorsJoseph L. Witztum, Sotirios Tsimikas, Xiaoli Sun
Life of this patent
Estimating the term…
Abstract
The disclosure provides for methods, compositions and kits that utilize total Oxidized phospholipids to determine whether a subject has liver disease.
Claims26
A method for determining and or distinguishing a disease or disorder associated with oxidized phospholipids (OxPL) in a subject, the method comprising:
- a) obtaining a sample from the subject;
- b) spiking the sample with labeled phosphocholine (labeled-PC) to obtain a spiked sample;
- c) contacting the spiked sample with a substrate comprising known quantity of an antibody, antibody fragment or non-immunoglobulin binding domains that bind to OxPL under conditions such that OxPL and labeled-PC bind to the known quantity of antibody, antibody fragment or non-immunoglobulin binding domains;
- d) washing the substrate to remove unbound OxPL and labeled-PC;
- e) contacting bound labeled-PC with an agent that measures the amount of labeled-PC;
- f) comparing the levels of labeled-PC to a standard curve;
- and
- g) identifying an amount of total OxPL in the sample based upon the standard curve, wherein the amount is indicative of a disease or disorder associated with OxPL.
5 more elements
A method for determining and distinguishing a liver disease associated with OxPL in a subject, the method comprising:
- a) obtaining a sample from the subject;
- b) spiking the sample with labeled phosphocholine (labeled-PC) to obtain a spiked sample;
- c) contacting the spiked sample with a substrate comprising a known quantity of antibody, antibody fragment or non-immunoglobulin binding domains that bind to OxPL under conditions such that OxPL and labeled-PC bind to the known quantity of antibody, antibody fragment or non-immunoglobulin binding domains;
- d) washing the substrate to remove unbound OxPL and labeled-PC;
- e) contacting bound labeled-PC with an agent that measures the amount of labeled-PC;
- f) comparing the levels of labeled-PC to a standard curve;
- and
- g) identifying an amount of total OxPL in the sample based upon the standard curve, wherein the amount is indicative of whether the subject has liver disease.
5 more elements
A method of monitoring an obesity/weight loss treatment in a subject, the method comprising:
- a) obtaining a plurality of samples from the subject prior to and during treatment;
- b) spiking the plurality of samples with labeled phosphocholine (labeled-PC) to obtain a spiked sample;
- c) contacting the spiked sample with a substrate comprising known quantity of an antibody, antibody fragment or non-immunoglobulin binding domains that bind to OxPL under conditions such that OxPL and labeled-PC bind to the known quantity of antibody, antibody fragment or non-immunoglobulin binding domains;
- d) washing the substrate to remove unbound OxPL and labeled-PC;
- e) contacting bound labeled-PC with an agent that measures the amount of labeled-PC;
- f) comparing the levels of labeled-PC to a standard curve;
- and
- g) identifying an amount of total OxPL in the sample based upon the standard curve, wherein the amount is indicative of a change in total OxPL in the subject during treatment.
5 more elements
Dependencies are inferred from the claim text.