Automatic test card for multi-blood group system and test method

A test card of a multi-blood group system, comprising four layers from top to bottom:

• a sample loading layer, a reaction layer, a color developing layer, and an identification layer;
• the sample loading layer being provided with a sample loading hole,
• the reaction layer being provided with a whole blood pad or a filter pad for filtering red blood cells (RBCs) from whole blood, and bottom of the sample loading hole being connected to the whole blood pad or the filter pad;
• an end of the whole blood pad being connected to a plurality of antibody pads pre-coated with blood group antibodies, or an end of the filter pad being connected to antigen pads pre-coated with blood group antigens via a delay pad;
• the reaction layer being independently provided with a quality control strip;
• wherein the color developing layer is provided with a plurality of color developing areas connected to an end of the antibody pad or the antigen pad;
• the identification layer is reversely connected to the color developing layer, and comprises three two-dimensional code positioning areas, a calibration area, a plurality of test areas, and a subject information area;
• and positions of the test areas and positions of the color developing areas being aligned with each other one-to-one
• such that the test areas overlaps with the color developing areas and provides a hollow hole or a transparent membrane on the test areas;
• and
• the identification layer has a shape of square, the three two-dimensional code positioning areas are distributed in three corners of the square, and the subject information area includes a QR two-dimensional code, a three-dimensional code, or a barcode used for registration and storage of subject information and is distributed in the fourth corner of the square;
• and the calibration area is composed of 6 color blocks, with RGB being (68, 114, 196), (240, 142, 24), (44, 220, 65), (218, 46, 148), (220, 78, 44), and (41, 219, 223).